National Technical University of Athens Mechanical Engineers
Department of Bioengineering
Special Thanks, i would like to thank all members of the lab for their help and especially George Kanakaris who helped me with his advice.
1) Micro-Beads & Bead-Based Sandwich ELISA:
Micro-Bead Trapping Device refers to magnetic micro-beads (typical diameter of about 5 microns) used in Bead-Based Sandwich ELISA. The microbeads contain 2-4% Fe which is responsible for their magnetic abilities.
In typical ELISA procedures we are able to identify and count one single protein at a time. In Bead-Based Sandwich ELISA the use of magnetic micro-beads adds multiplexability which means we are able to identify up to 100 different proteins (in reality practical reasons prevent us from doing so).
How?
The beads are identified by a fluorescent dye, being impregnated with the mixture of red and infrared dyes in 10 different concentrations each. Therefrom 100 different colour identities are feasible.
MICRO-BEAD COLOUR LABEL
BEAD-BASED SANDWICH ELISA
2) Device Specifications
The beads along with the solvent containing the proteins is put inside an eppendorf tube.
Eppendorf tubes must touch permanent magnets so that the beads stick to the surface, therefore being able to change the solvent according to the ELISA procedures.
The device must be able to adjust to a shaker as well as to a shonicator and float. Both the shaker and the sonicator are used to facilitate the diffusion processes. Sonication is also needed to split the beads (magnetic beads have the tendency to form sets of beads tight to each other-obviously we don't want this in our experiments).
The pippet's tips -used to remove the solvents from the eppendorf tubes- are spaced 9cm. Eppendorf tube's diameter is about 10.7mm therefore eppendorf tubes must be spaced 18mm.
LAB EQUIPMENT- PIPETTE TIP & EPPENDORF TUBE
LAB EQUIPMENT-SONICATOR
LAB EQUIPMENT-PIPETTE
LAB EQUIPMENT-SHAKER (VORTEX)
3) ALTERNATIVE CONCEPT DESIGNS
CONCEPT DESIGN 1
CONCEPT DESIGN 2
CONCEPT DESIGN 3
The designs presented above are only displayed to show different aspects of the designing process
During the designing process aspects of the exact constraint theory were used.
4) FINAL DESIGN
Micro-Bead Trapping Device
Main device body with float
I may alter the float's geometry should we decide that the float is to be consumable.
UPPER PLATE
FRAME
MIDDLE PLATE
5) MATERIALS USED
Upper plate & frame: compact polycarbonate sheet
Legs, dowel pins, lower plate and middle part : aluminum alloys 2011 T6 and 7075 T651
Float: closed-cell PU foam, density=0.037g/cm^3
6) PROCESSES
Legs & dowel pins: turning
Upper plate, frame & lower plate: waterjet cutter
Middle part: cnc milling and drilling
Numbers on upper plate: engraving
Specifically for the middle part, lacking a 4th axis on the lab's cnc milling center we used a special clamp.
The clamp is demonstrated below.
MICROBEAD TRAPPING
2012-2013
Microbead Trapping Device:
Vasileiou Georgios
(georgiosvasileiou@hotmail.com)
LinkedinNational Technical University of Athens
Mechanical Engineers
Department of Bioengineering
Special Thanks,
i would like to thank all members of the lab for their help and especially George Kanakaris who helped me with his advice.
1) Micro-Beads & Bead-Based Sandwich ELISA:
Micro-Bead Trapping Device refers to magnetic micro-beads (typical diameter of about 5 microns) used in Bead-Based Sandwich ELISA. The microbeads contain 2-4% Fe which is responsible for their magnetic abilities.
In typical ELISA procedures we are able to identify and count one single protein at a time. In Bead-Based Sandwich ELISA the use of magnetic micro-beads adds multiplexability which means we are able to identify up to 100 different proteins (in reality practical reasons prevent us from doing so).
How?
The beads are identified by a fluorescent dye, being impregnated with the mixture of red and infrared dyes in 10 different concentrations each. Therefrom 100 different colour identities are feasible.
2) Device Specifications
3) ALTERNATIVE CONCEPT DESIGNS
The designs presented above are only displayed to show different aspects of the designing process
During the designing process aspects of the exact constraint theory were used.
4) FINAL DESIGN
I may alter the float's geometry should we decide that the float is to be consumable.
5) MATERIALS USED
Upper plate & frame: compact polycarbonate sheet
Legs, dowel pins, lower plate and middle part : aluminum alloys 2011 T6 and 7075 T651
Float: closed-cell PU foam, density=0.037g/cm^3
6) PROCESSES
Specifically for the middle part, lacking a 4th axis on the lab's cnc milling center we used a special clamp.
The clamp is demonstrated below.